| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Central Minnesota Youth Soccer Association Central Minn - B16 |
0WCMS-CMSBAB16-0074 | Gabriel Monaighan |
| A2 | Sartell Soccer Association Sartell Boys 16U |
0WSSR-SSRBAB16-0007 | Brandon Testa |
| A3 | Willmar Soccer Association Willmar Socc - BA16 |
0WWIL-WILBAB16-0045 | Francis Winter |
| A4 | Great River Soccer Club Great River - B16 - Fisher |
0WGRV-GRVBAB16-0118 | Tanner Fisher |
| A5 | CC United Soccer Club CC United Boys 16U Black |
0WCCU-CCUBAB16-0132 | Roland Capehart |
| A6 | Waconia Soccer Club Waconia Soccer Club - Krugerud - B16 |
0WWAC-WACBAB16-0137 | Joshua Krugerud |
| A7 | Inver Grove Heights Soccer Association IGH Heat - B16 |
0EIGH-IGHBAB16-0121 | Jacob Myran |
| A8 | Minneapolis United Soccer Club MUS 16U Boys Samba |
0WMUS-MUSBAB16-0160 | Juan Andrade-Vera |
| A9 | Joy of the People Joy of the P - B16 |
0EJOP-JOPBAB16-0005 | n/a |
| B1 | New Ulm United Soccer Club New Ulm United Soccer Club - B16 |
SWNUS-NUSBAB16-0044 | Joshua Kamrath |
| B2 | Worthington FC Worthington FC - B16 |
SWWFC-WFCBAB16-0010 | Juan Ignacio |
| B3 | Mankato United Soccer Club Mankato Unit - B16 |
SWMNK-MNKBAB16-0112 | Lukas Dang |
| B4 | Austin Youth Soccer Association Austin Boys 16U Black |
0SAUS-AUSBAB16-0005 | Michael Clausman |
| B5 | Mississippi Valley Soccer Club Mississippi Valley Soccer - B16 Black |
0SMVS-MVSBAB16-0040 | Jason Mahlum |
| B6 | Marshall United Soccer Association Marshall - B16 |
SWMAR-MARBUB16-0001 | Kelly Schuerman |