| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Lakeville Soccer Club Lakeville SC B04 Select |
0SLKV-LKVDB16-1110 | Michael Hillis |
| A2 | Mankato United Soccer Club Mankato Unit - B16 |
SWMNK-MNKDB16-0002 | Clayton Merches |
| A3 | Keliix-Intra Soccer Club Keliix-Intra - B16 |
0WKLX-KLXDB16-0007 | Sean Baker |
| A4 | Eau Claire United Soccer Club EauClaireUnited 2004B |
WIECU-ECUDB16-0048 | Aaron Hanson |
| A5 | Minnesota Rush Minnesota Rush - B16 |
0SMNR-MNRDB16-0126 | Frank Spaeth |
| A6 | Force Soccer Club Force - 16U Boys League One |
0NFSC-FSCDB16-0016 | n/a |
| A7 | Orono-Westonka Soccer Club Orono-Weston - B16 League 1 |
0WOWS-OWSDB16-8611 | Brian Grand |
| A8 | CC United Soccer Club CC United Boys 16U Div1 |
0WCCU-CCUDB16-0017 | Kevin Carlson |
| B1 | Manitou F.C. White Bear SC 2004 Boys Orange |
0EWBL-WBLDB16-2254 | Andrew Lynch |
| B2 | Z - MN Eclipse MapleBrook Soccer Club Maplebrook BU16 - Paye |
0N001-001DB16-0032 | Peter Paye Jr |
| B3 | Richfield Soccer Association Richfield Soccer Association - X16 |
0SRFL-RFLDB16-0141 | Angel Dominguez Reyes |
| B4 | Minnesota Rush Minnesota Rush - B16 |
0SMNR-MNRDB16-0125 | Humberto Paulo |
| B5 | Minneapolis United Soccer Club MUS 16U Boys Copa |
0WMUS-MUSDB16-0122 | Zachary Neiberger |
| B6 | Twin Cities Rush Soccer Club Blaine 04B EDT1 |
0NBLA-BLADB16-0411 | Sebastien Dixon |
| B7 | Blackhawks Soccer Club Blackhawks 04 B Red |
0EBHK-BHKDB16-0099 | Ahmed Charai |
| B8 | Fusion Soccer Club Fusion Boys 04 Select 2 |
0WFUS-FUSDB16-0035 | Kent Getchell |