| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Manitou F.C. White Bear SC 2004 Girls Black |
0EWBL-WBLBAG16-2262 | Jonathan Staruck |
| A2 | Z - Three Rivers Soccer Association NMTR G 2004 BA |
0NTRV-TRVBAG16-0013 | Liam Townsend |
| A3 | St. Anthony Soccer Club St. Anthony Soccer Club - G16 |
0NSAN-SANBAG16-0040 | Thomas Metcalf |
| A4 | Southeast Soccer Club SES U16G Stingrays |
0ESES-SESBAG16-0048 | Frank Clancy |
| A5 | Minneapolis United Soccer Club MUS 16U Girls Samba |
0WMUS-MUSBAG16-0143 | Hana Mohammad |
| A6 | Coon Rapids Soccer Association CRU Girls 2004 Black |
0NCRA-CRABAG16-0017 | NickDuplicate KoppDuplicate |
| A7 | Z - MN Eclipse MapleBrook Soccer Club Maplebrook GU16 Black |
0N001-001BAG16-0006 | Deleted Deleted |
| A8 | Northern Lights Soccer Club Northern Lights Soccer Club - 0NNLS - G16 |
0NNLS-NLSBAG16-0002 | Lisa Hansen |
| A9 | St. Michael-Albertville Soccer Club STMA Christensen |
0WSTM-STMBAG16-0166 | n/a |
| B1 | Alexandria Area Soccer Association Alexandria Area Soccer Association - G16 |
NWALX-ALXBAG16-0054 | n/a |
| B2 | Arrowhead Youth Soccer Association Arrowhead Cloquet 16UG Black League |
0NAHD-AHDBAG16-2010 | Bobbie Turner |
| B3 | Cambridge-Isanti Soccer Club Cambridge-Is - G16 |
0NCIS-CISBAG16-0056 | Craig Nystrom |
| B4 | Central Minnesota Youth Soccer Association Central Minn - G16 BA |
0WCMS-CMSBAG16-0021 | Cassandra Evans |
| B5 | Great River Soccer Club Great River - G16 - Skoog |
0WGRV-GRVBAG16-0077 | Brandon Skoog |
| C1 | Richfield Soccer Association Richfield - G16 |
0SRFL-RFLBAG16-0151 | Stanley Luke |
| C2 | Waconia Soccer Club Waconia Socc - G16 |
0WWAC-WACBAG16-0125 | Lonnie Fette |
| C3 | Mankato United Soccer Club Mankato Unit - G16 |
SWMNK-MNKBAG16-0010 | Emma Morris |
| C4 | New Prague Soccer Club New Prague S - G16Blk |
0SNPS-NPSBAG16-0006 | Jason Schwakopf |
| C5 | Northfield Soccer Association Northfield S - G16 |
0SNFD-NFDBAG16-0001 | Brent Kivell |